实验动物科学与实验医学 | AMEM 2020年高被引文章集锦
《动物模型与实验医学（英文）》（Animal Models and Experimental Medicine，AMEM）由中国实验动物学会、中国医学科学院医学实验动物研究所共同主办，是一本注重学科交叉、注重成果转化、引领实验动物科学及实验医学发展的OA期刊。本期，小编精选了AMEM于2020年出版的Top 10高被引文章，欢迎大家免费阅读和分享~
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SHORT COMMUNICATION
Age-related rhesus macaque models of COVID-19年龄相关的COVID-19恒河猴模型作者：Pin Yu,Feifei Qi,Yanfeng Xu,Fengdi Li,Peipei Liu,Jiayi Liu,Linlin Bao,Wei Deng,Hong Gao,Zhiguang Xiang,Chong Xiao,Qi Lv,Shuran Gong,Jiangning Liu,Zhiqi Song,Yajin Qu,Jing Xue,Qiang Wei,Mingya Liu,Guanpeng Wang,Shunyi Wang,Haisheng Yu,Xing Liu,Baoying Huang,Wenling Wang,Li Zhao,Huijuan Wang,Fei Ye,Weimin Zhou,Wei Zhen,Jun Han,Guizhen Wu,Qi Jin,Jianwei Wang,Wenjie Tan,Chuan Qin
In late December of 2019, a cluster of severe pneumonia cases caused by severe acute respiratory syndrome coronavirus (SARS-CoV-2) were reported from Wuhan, China. It is critical to understand the pathogenicity of this virus for the prevention and treatment of Coronavirus disease 2019 (COVID-19). SARS-CoV-2 was isolated and classified as Betacoronavirus, belonging to the lineage B or subgenus Sarbecovirus, which also includes the human SARS coronavirus. The full virus genome of SARS-CoV-2 has about an 89% nucleotide identity with bat-SL-CoVZC45, the Spike protein has a 78% nucleotide identity with the human SARS coronavirus, and an 84% nucleotide identity with the bat-SL-CoVZC45 coronavirus. The SARS-infected rhesus monkey model was once established, and now the SARS-CoV-2-infected rhesus monkey model can dynamically understand the pathogenicity of COVID-19.
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GUIDELINES AND CONSENSUS
Guidelines for the ethical review of laboratory animal welfare People’s Republic of China National Standard GB/T 35892-2018实验动物福利伦理审查指南中华人民共和国国家标准GB/T 35892-2018作者：Judy A. MacArthur Clark, Deming Sun
These Chinese National Guidelines (GB/T 35892-20181) were issued February 06, 2018 and became effective September 01, 2018. The authors recognized the urgent need for an authentic English translation to inform the international community of the compliance requirements in China. It was appreciated that the final translation must reflect the specialist understanding of those working under the Guideline whilst remaining faithful to the meaning of the original Chinese text. A three-step translation process was therefore determined.
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ORIGINAL ARTICLE
The role of mouse models in colorectal cancer research-The need and the importance of the orthotopic models小鼠模型在结直肠癌研究中的作用——原位模型的必要性和重要性作者：Rui C. Oliveira, Ana Margarida Abrantes, José Guilherme Tralhão, Maria Filomena BotelhoColorectal cancer (CRC) is a major medical concern, being the third most common cancer type and the fourth most common cause of cancer-related death, accounting for 9% of the total cancer incidence. In spite of the progress in clinical and biological knowledge, CRC remains a main public health issue, and despite the rapid development of treatments in the last years, the mortality rate related to this type of cancer remains high,especially in the advanced stages of disease. About half of the patients with CRC will develop liver metastases, and from that subpopulation only 25% are eligible for surgery with curative intent.
DOI: https://doi.org/10.1002/ame2.12102
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ORIGINAL ARTICLE
Optimization of food deprivation and sucrose preference test in SD rat model undergoing chronic unpredictable mild stress慢性不可预测性应激SD大鼠模型中食物剥夺和糖水偏爱实验的优化
作者：Li-Wen He, Li Zeng, Na Tian, Yi Li, Tong He, Dong-Mei Tan, Qian Zhang, Yi Tan
Current animal models of depression are mainly divided into three categories: stress models, surgical models, and chemical drug-induced models. The most commonly used animal model is the chronic unpredictable mild stress (CUMS) model, which is the only model which manifests high surface, structural, and predictive validity. However, the CUMS model has been questioned for its poor repeatability, most likely due to variation in stimulus modes, intensity and frequency. Establishment of the CUMS model generally consists of two stages. The first stage is the exposure of the animals to noxious stimuli such as a tilted cage or food/water deprivation for long periods; and the second stage is the use of behavioral tests to screen the animals exhibiting depression-like behavior.
DOI: https://doi.org/10.1002/ame2.12107
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ORIGINAL ARTICLE
Knock in of a hexanucleotide repeat expansion in the C9orf72 gene induces ALS in rats在C9orf72基因中敲入六核苷酸重复扩增会诱导大鼠的肌萎缩性侧索硬化症作者：Wei Dong, Li Zhang, Caixian Sun, Xiang Gao, Feifei Guan, Jing Li, Wei Chen, Yuanwu Ma, Lianfeng Zhang
The GGGGCC (G4C2) repeat expansion in the human open reading frame 72 on chromosome 9, C9orf72, is the most common cause of amyotrophic lateral sclerosis (ALS). Studies in transgenic mouse models have linked the pathogenic mechanism of G4C2 repeat expansion to RNA foci or the accumulation of unnatural dipeptide repeats in neurons. However, only one of the existing transgenic mouse lines developed typical ALS.
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ORIGINAL ARTICLE
Assessing neuraxial microstructural changes in a transgenic mouse model of early stage Amyotrophic Lateral Sclerosis by ultra-high field MRI and diffusion tensor通过超高场MRI和弥散张量指标评估早期肌萎缩性侧索硬化症转基因小鼠模型中的神经轴微观结构变化作者：Rodolfo G. Gatto, Carina Weissmann, Manish Amin, Ariel Finkielsztein, Ronen Sumagin, Thomas H. Mareci, Osvaldo D. Uchitel, Richard L. Magin
The introduction of animal models has been one of the major steps forward towards a better understanding of the neuropathological processes occurring in humans. Based on their similarity to the human genome and easy availability, mammalian murine models have been one of the most commonly used representations of neurodegenerative diseases such as Amyotrophic Lateral Sclerosis (ALS). As such, the phenotypical expression and histological discoveries parallel many of the symptomatology and neuropathological findings observed in patients with ALS.During the last decades, the exponential growth of novel genetic tools has led to the detection of new mutations in the patient population, and thus to help in the development of new transgenic animal models for research and therapeutic discovery purposes.
DOI: https://doi.org/10.1002/ame2.12112
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REVIEW ARTICLE
Impact of host genetics on gut microbiome: Take-home lessons from human and mouse studies宿主遗传学对肠道微生物组的影响：从人类和小鼠研究中学习作者：Inbal Cahana, Fuad A. Iraqi
The intestinal microbiome has emerged as an important component involved in various diseases. Therefore, the interest in understanding the factors shaping its composition is growing. The gut microbiome, often defined as a complex trait, contains diverse components and its properties are determined by a combination of external and internal effects. Although much effort has been invested so far, it is still difficult to evaluate the extent to which human genetics shape the composition of the gut microbiota. However, in mouse studies, where the environmental factors are better controlled, the effect of the genetic background was significant. The purpose of this paper is to provide a current assessment of the role of human host genetics in shaping the gut microbiome composition. Despite the inconsistency of the reported results, it can be estimated that the genetic factor affects a portion of the microbiome. However, this effect is currently lower than the initial estimates, and it is difficult to separate the genetic influence from the environmental effect. Additionally, despite the differences between the microbial composition of humans and mice, results from mouse models can strengthen our knowledge of host genetics underlying the human gut microbial variation.
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ORIGINAL ARTICLE
Epalrestat improves motor symptoms by reducing oxidative stress and inflammation in the reserpine induced mouse model of Parkinson’s disease依帕司他通过减少利血平诱导的帕金森病小鼠模型氧化应激和炎症改善运动症状作者：Md. Mahbubur Rahman, Rupali Rani Chakraborti, Md. Abdullah Potol, Ariful Haque Abir, Ozayra Sharmin, Mahabub Alam, Md. Fazlur Rahman Khan, Rownock Afrin, Humayra Jannat, Rasiqh Wadud, Zaki Farhad Habib
Currently, Epalrestat is indicated for the management of diabetic neuropathy. It improves the antioxidative defense mechanism in the CNS. Through transcriptional regulation, Epalrestat increases intracellular GSH levels. Decreased GSH levels are common long-term complications in patients who have diabetes. It has been found that Epalrestat improves morphological abnormalities of nerves in the rodent model of diabetes. One study explored the effect of Epalrestat in a cell culture model of oxidative stress, but little is known about its effects on the in vivo system, in particular the phenotypic outcome of its use in neurodegenerative disorders like PD.28 Therefore, in our current study, we evaluated the role of Epalrestat in a mouse model of PD.
DOI: https://doi.org/10.1002/ame2.12097
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ORIGINAL ARTICLE
Curculigo recurvata WTAiton exhibits anti-nociceptive and anti-diarrheal effects in Albino mice and an in silico model大叶仙茅在白化病小鼠和生物信息学模型中表现出抗痛觉和抗腹泻作用作者：Shabbir Ahmad, Mst. Samima Nasrin, A. S. M. Ali Reza, Nishan Chakrabarty, Md. Akramul Hoque, Sanjida Islam, Mohammad Shah Hafez Kabir, Syed Mohammed Tareq, A. H. M. Khurshid Alam, Md. Areeful Haque, Md. Saiful Islam Arman
Curculigo recurvata (C. recurvata) W.T.Aiton (Family Hypoxidaceae) is a perennial herb found in the Chittagong Hill Tracts, and available in Cox's Bazar, Moulvibazar, and Sylhet in the marginal forest areas of Bangladesh. The genus, Curculigo, comprises 20 species. Some of these species are used by traditional practitioners and are believed to have significant medicinal values. The rhizomes of C. recurvata (RCR) are widely used in traditional medicine for several ailments such as snake bites, consumptive cough, impotence, asthma, jaundice, diarrhea, colic, gonorrhea, and arthropod stings, as well as for the treatment of menoxenia, bleeding disorders, nephritis, arthritis, and leucorrhea. The traditional uses of RCR have been validated by animal and clinical studies, including hypoglycemic, antibacterial, anthelmintic, antithrombotic, and cytotoxic studies. Mustakim et al, for example, confirmed that a crude methanol extract of C. recurvata rhizomes possesses potential antioxidative and cytotoxic properties. Two novel diastereoisomer glucosides, curculigine and isocurculigine, have been identified from this species, and glucosides of curculigo rhizome have been shown to significantly improve premenopausal syndrome, characterized by hysteria, depression, and melancholia. Accumulating evidence suggests that other species of the Curculigo genus possess various therapeutic effects. For instance, the C. orchioides has shown neuroprotective, anticancer, antiosteoporotic, immunostimulatory, and estrogenic activities.
DOI: https://doi.org/10.1002/ame2.12119
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ORIGINAL ARTICLE
Supplements of an aqueous combination of Justicia adhatoda and Ocimum tenuiflorum boost antioxidative effects and impede hyperlipidemiaJusticia adhatoda 和Ocimum tenuiflorum水提混合物可促进抗氧化，降血脂作者：Ifrahim Iqbal Chowdhury, Md. Atiar Rahman, Mohammad A. Hashem, M. Mosharef Hossain Bhuiyan, Dina Hajjar, Walla Alelwani, Arwa A. Makki, Md. Areeful Haque, Jitbanjong Tangpong, M. Taher Bin Bakhtiar
Justicia adhatoda and Ocimum tenuiflorum, locally known as bashok and tulsi, are two ethnomedicinally important herbs that have been using as dietary supplements for several therapeutic applications. This study evaluated the combined effect of both the herbs as an antioxidative and antihyperlipidemic agent.
The results showed that the serum lipid profile was significantly decreased in the different treatment groups, with bashok having the greatest effect. Body weights, total serum protein, LDH, and relative liver and adipose tissue weights were markedly restored towards baseline values, the lowest atherogenic index being achieved with the combined extract. The combination treatment significantly enhanced total phenolic content and antioxidative capacity and greatly potentiated membrane stabilization, but inhibition of protein denaturation was not significantly affected.
DOI: https://doi.org/10.1002/ame2.12115
期刊简介
《动物模型与实验医学（英文）》（Animal Models and Experimental Medicine，AMEM）由中国实验动物学会、中国医学科学院医学实验动物研究所共同主办，与国际著名出版集团Wiley合作出版。AMEM已被DOAJ，PMC, CSCD, MEDLINE，ESCI收录，作者和读者遍布中国、 美国、印度、英国、德国、日本、加拿大等世界各地。
AMEM围绕实验动物科学基础和应用研究，聚焦实验动物物种资源、人类疾病动物模型资源、比较医学资源等，展示实验动物科学及实验医学的最新学术、理论、技术、应用成果，以及实验动物在生命科学、医学、生物学、药学、兽医学等交叉学科中的实验研究，拓展实验动物相关设备材料的研发、实验动物管理与法律标准、实验动物福利伦理等新思路，是一本注重学科交叉、注重成果转化、引领实验动物科学及实验医学发展的英文专业期刊。
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